BRCA 1/2 gene mutations do not affect the capacity of cumulo-oocyte-complexes to mature in vitro in breast cancer candidates for fertility preservation

Abstract

Mutation in BRCA1 and BRCA2 genes are associated with an increased risk for developing breast and ovarian cancer. Controversy exists about ovarian reserve and fertility in BRCA mutation carriers. Studies suggest that these patients may have low ovarian reserve and poor response to ovarian stimulation. The impaired ability of the mutated BRCA gene to repair double-strand breaks in DNA may prompt oocyte aging, apoptosis and meiotic errors. In vitro maturation (IVM) of cumulo-oocyte complexes (COC) retrieved at germinal vesicle stage, followed by vitrification of metaphase 2 oocytes, has recently emerged as an option for young women seeking fertility preservation, when ovarian stimulation is unfeasible.