Abstract
Aim To prove brazilein's effect on 5-HT receptor and to understand the characteristics of the action. Methods In this work, the blocking effect of brazilein on 5-HT receptor subtypes was investigated in isolated uterine strips using pharmacological (selective agonists) and molecular biological (real time PCR) approaches. Results brazilein does not affect the contractions of normal mice uterus, but inhibits uterine excitatory elicited by ergometrine maleate (pA2 = 5.71 ± 0.21), a 5-HT 2 receptor agonist. The excitatory effects of 5-HT 1D, 5-HT 2A and 5-HT 2C receptor agonists on uterus could be inhibited by brazilein (pA2 = 4.58 ± 0.06, 4.91 ± 0.15, 5.38 ± 0.15, respectively). The mRNA expression of 5-HT 1D and 5-HT 2C receptors in mice uterus were decreased by brazilein. Conclusion brazilein blocked 5-HT receptors in mice uterus and might affect the functions mediated by 5-HT receptors.
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