Brassinosteroid-induced de novo protein synthesis in Zea mays under salinity and bioinformatic approach for identification of heat shock proteins

Abstract

Using radioactive labeling technique it was analyzed proteins profiles of maize plants after treatment (12 h) with salt and brassinosteroids (BRs). Among separated proteins 66 and 67 proteins were observed specifically upregulated by salt treatment or BRs under salinity, respectively. Brassinazole—inhibitor of BRs biosynthesis—lowered BR-induced accumulation of proteins that confirm their BR-mediated synthesis. Data on 200 heat shock proteins (HSPs) from Arabidopsis thaliana and 42 HSPs from Zea mays have been used for polypeptide identification with bioinformatic approach. Protein bands separated on SDS-PAGE were compared with gene expression profile of HSPs from Genevestigator database in response to salinity and hormone treatment. In result, eight correlative matches were found between levels of polypeptide accumulation and activation of HSPs genes’ expression. It was suggested involvement of BRs in regulation of synthesis of mitochondrial chaperones under salinity.