Abstract
Nitrate is the major nutrient form of nitrogen absorbed by plant from soil. It was reported that high nitrate level in the leaves of leafy vegetables may be harmful to human health, thus it was concerned to be very important on the nitrate level of leaves of kale for their large amount of consumption in Taiwan. The object of the study was to investigate the effect of sampling time and shading on the nitrate concentration and nitrate reductase activity of the shoot of kale by solution culture. There were no significant effect of sampling time and shading on reduced nitrogen level of the leaf, petiole, stem and root of kale in solution culture with 7mM NO3^-and 1mM NH4^+ as its N-source. Stopping supplying nitrate gradually decreased the nitrate level of the kale. The nitrate concentration of fresh leaf was the half of the of petiole and stem, however, nitrate reductase activity of leaves was ten times of that of petiole and stem.
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