Abstract
The branching was observed to occur in fern gametophytes cultured in various light conditions (strong white light of 2, 500 lux, red light and darkness). The nuclear volume, amounts of total nitrogen and total nucleic acid phosphorus, and other cytochemically detected elements suggested strongly a distinct apico-basal gradient of the physiological activity in the gametophyte, and the growth was carried out only at the apical region likely of the greatest activity. Light conditions as mentioned above seem to destroy this gradient, to alter the developmental axis and consequently to induce the branching.
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