Abstract
A simplified assay for tryptamine (TRYP) by gas chromatographymass fragmentography (GC-MF) is described. TRYP was analyzed as its N-acetyl-1-trifluoroacetyl derivative. Acetylation of TRYP was carried out at basic pH using acetic anhydride dissolved in a diethyl ether vehicle. N-acetyl-TRYP was extracted into the diethyl ether from a basic aqueous phase during the acetylation reaction. TRYP-D 2 was employed as an internal standard. TRYP was quantitatively assayed in human urine and rat brain using multiple ion detection GC-MF. Assay sensitivities of 500 pg could be achieved by single ion and 5 ng by double ion GC-MF. In rats treated with pargyline and pargyline plus tryptophan, brain TRYP was found to be 35.6 ± 4.9 (mean ± SE) and 133 ± 3.8 ng/g, respectively. However, TRYP was not detected in normal rat brain at an assay sensitivity of 500 pg.
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