Brain temperature- and behavior-related changes in the dentate gyrus field potential during sleep, cold water immersion, radiant heating, and urethane anesthesia

Abstract

The field potential evoked in the dentate gyrus (DG) by stimulation of the perforant path (PP) is known to vary with ongoing behavior and with brain temperature. To further study these phenomena chronic stimulating and recording electrodes were implanted into the PP and DG of rats, and a thermistor was implanted into the contralateral homotopic DG. Field potentials and brain temperature records were made during (1) slow wave sleep (SWS), (2) radiant heating, (3) immersion in cool water, (4) a control session during which no manipulations were made, and (5) under urethane anesthesia. In another group of rats field potentials were recorded during (1) baseline immobile wakefulness, (2) SWS, (3) before SWS or after gentle awakening from SWS (eyes open and presence of intermittent slow waves in the EEG), (4) immobile wakefulness, and (5) 24 h later. Findings were that field EPSP slope decreased and population spike (PS) amplitude increased by up to 60% of baseline values during conditions in which brain temperature was reduced (SWS, immersion in cool water, urethane anesthesia). Conversely, EPSP slope increased and PS amplitude decreased by up to 100% of baseline values during conditions in which brain temperature increased (awakening from SWS, radiant heating, and warming after immersion in cool water or urethane anesthesia). Product moment correlations between brain temperature and field potential measures confirmed the statistical reliability of these findings and accounted for up to 77% of the variance. These findings confirm the robust effect on hippocampal field potentials of brain temperature changes due to exogenous heating and cooling, and extend this effect to anesthetic- and sleep-induced brain temperature changes. They also identify a state that behaviorally resembles quiet wakefulness but resembles SWS in terms of neocortical EEG, brain temperature, and hippocampal field potential measures. The findings indicate the need to control for brain temperature-mediated changes in hippocampal research that uses the dentate gyrus field potential as a dependent measure.