Abstract
This study was conducted to screening for Myxobolus sp . infections in the brain of African sharptooth catfish (Clarias gariepinus). A total of 120 fish were collected over one year from Al fath center in Assiut Governorate. Smears of all samples were examined parasitologically, and polymerase chain reaction (PCR) was used to confirm selected positive samples. Examination revealed the presence of Myxobolus sp . in 57 (47.5%) of examined fish. Prevalence of infection was highest in winter and spring (56.7%) and became low in autumn (30%). The effect of body weight revealed that, the highest prevalence rate (52.9 %) was occurred in middle group (301- 400g). Female fish seems to be more sensitive to infection (48.5%) than male (46.2%). Morphological characters and measurements of mature spores were recorded. The amplified 18S rDNA gene fragment by using a specific primer for Myxosporean was (869bp).
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