Abstract
BackgroundThe incidence of thyroid cancer is increasing worldwide. This study investigated the association of B-type RAF kinase (BRAF)V600E mutation status, the expression of BRAF-activated long non-coding RNA (BANCR) and microRNA miR-9, and the clinicopathological features of papillary thyroid carcinoma (PTC).MethodsClinicopathological data for PTC patients (n = 51) diagnosed and treated between 2018 and 2019 were collected. Carcinoma and adjacent normal tissue samples were analyzed for the presence of the BRAFV600E mutation and/or expression of BANCR and miR-9.ResultsLarger tumor, higher rate of bilateral tumors and multifocality, extracapsular invasion, and lateral lymph node metastasis (LNM) were observed in PTC patients with BRAF V600E mutation. Patients with higher BANCR expression had a higher rate of extracapsular invasion and lateral LNM in carcinoma tissue and a lower frequency of bilateral tumors and multifocality in normal adjacent tissue. Patients with higher miR-9 expression had a lower rate of central and lateral LNM in carcinoma tissue and higher rates of bilateral tumor location and multifocality in normal adjacent tissue. Patients with BRAFV600E mutation have a higher rate of BANCR overexpression and tended to have a lower rate of miR-9 overexpression (P = 0.057), and a negative association was observed between BANCR and miR-9 expression in carcinoma tissue.ConclusionsBRAFV600E mutation and the BANCR and miR-9 expression were closely associated with the tumor size, bilateral tumor location, multifocality, extracapsular invasion, and lateral LNM. PTC patients with these clinicopathological characteristics, BRAFV600E mutation, and high BANCR expression and low miR-9 expression needed earlier surgical treatment and are recommended for total thyroidectomy in primary surgery for reducing the risk of recurrence. These findings provide new insight into the molecular basis for PTC and can inform strategies for the management of PTC.
Highlights
Thyroid cancer is an endocrine malignancy and its incidence is increasing worldwide, especially in developed countries [1]
BRAFV600E mutation and the BRAF-activated long non-coding RNA (BANCR) and miR-9 expression were closely associated with the tumor size, bilateral tumor location, multifocality, extracapsular invasion, and lateral lymph node metastasis (LNM)
We investigated the relationships between the molecular features of papillary thyroid carcinoma (PTC) including BRAFV600E mutation and BANCR and miR-9 expression, and clinicopathological characteristics of PTC patients
Summary
Thyroid cancer is an endocrine malignancy and its incidence is increasing worldwide, especially in developed countries [1]. The most common genetic changes in PTC are mutations in B-type RAF kinase (BRAF) of the RAS/BRAF/mitogen-activated protein kinase signaling pathway [3]; the most frequent mutation (95%) is BRAFV600E [4, 5], which is associated with enhanced extrathyroid extension, lymph node metastasis (LNM), and advanced tumor stage [6,7,8]. This has not been corroborated by other studies [9], and the relationship between clinicopathological characteristics of PTC and BRAFV600E mutation remains unclear. This study investigated the association of Btype RAF kinase (BRAF)V600E mutation status, the expression of BRAF-activated long non-coding RNA (BANCR) and microRNA miR-9, and the clinicopathological features of papillary thyroid carcinoma (PTC)
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