Bradyrhizobium Inoculation of Field-Grown Kersting's Groundnut [Macrotyloma geocarpum (Harms) Marechal &amp; Baudet] Increased Grain Yield and N2 Fixation, Measured Using the Ureide, and 15N Natural Abundance Techniques

Mustapha Mohammed,Felix D Dakora,Elias N K Sowley,Glory C Mbah

doi:10.3389/fsufs.2021.672247

Abstract

Here, we report on the symbiotic N2 fixation and water use efficiency (δ13C) of Kersting's groundnut, an important but underutilized grain legume in Africa, in response to inoculation with Bradyrhizobium strains 3267 and CB756. The 15N natural abundance and xylem ureide techniques were used to quantify N2 fixation. The landraces in this study derived variable levels of their N requirements from symbiosis, which translated into marked differences in the amounts of N-fixed by the landrace–inoculant combinations across the test locations. In most instances, the landrace-strain combinations that elicited higher shoot biomass also recorded greater N-fixed and/or ureide-N in xylem sap as well as grain yield. Although some landraces coupled increased grain yield with higher water use efficiency (shoot δ13C), a trait that could be tapped for crop improvement, others recorded lower yields despite eliciting relatively high shoot δ13C values, indicating genotypic variations in adaptation to the different environments. Grain yield of the test landraces showed marked variation and ranged from 131 to 1349.8 kg ha−1 depending on the landrace–strain combination used and the planting location. The high symbiotic dependence by landraces in this study could explain why Kersting's groundnut thrives in the low nutrient soils that are prevalent in its cultivation areas. These results provide more insights into the literature regarding the Kersting's groundnut–rhizobia symbiosis as well as the crop's water use efficiency.

Highlights

The N2-fixing trait of legumes offers them advantage over other species in N-limiting soils as they can reduce atmospheric N2 into useable forms for plants, through their symbiotic association with rhizobia (Menge and Chazdon, 2016)
Main Effect of Landrace on Plant Growth, Symbiotic Parameters, Grain Yield and Shoot δ13C A two-way ANOVA of data from Nyankpala showed that the main effect of landrace was significant (p ≤ 0.05) for nodule dry matter, shoot biomass, and N content at the site (Table 1)
Landraces Heng Red Mottled (Heng RM), Boli, Dowie, and Heng Milk Mottled (Heng MM) recorded significantly higher nodule mass compared to the other landraces at Nyankpala (Table 1)

Summary

Introduction

The N2-fixing trait of legumes offers them advantage over other species in N-limiting soils as they can reduce atmospheric N2 into useable forms for plants, through their symbiotic association with rhizobia (Menge and Chazdon, 2016). Strain CB756 and exhibit nitrate tolerant symbiosis in sand culture (Dakora, 1998), little has been done on the N2-fixing potential of Kersting’s groundnut under field conditions (Mohammed et al, 2018). This is despite the current efforts at harnessing the benefits of symbiotic N2 fixation for agriculture as well as the renewed interest in rhizobial inoculant production and usage (Stephens and Rask, 2000; Catroux et al, 2001; Hungria et al, 2006; Rebah et al, 2007)

Methods

Results

Discussion

Conclusion