Abstract
In NG 108-15 cells, bradykinin (BK) activates a potassium current (I K,BK) and inhibits the voltage-dependent calcium current (I Ca,V). BK also stimulates a phosphatidylinositol-specific phospholipase C (PI-PLC). The subsequent release of inositol 1,4,5-trisphosphate and increase in intracellular calcium contribute to I K,BK, through activation of a calcium-dependent potassium current. In membranes from these cells, stimulation of PI-PLC by BK is mediated by G q and/or G 11 two homologous, pertussis toxin-insensitive G proteins. Here, we have investigated the role of G q/l1 in the electrical responses to BK. GTPγS mimicked and occluded both actions of BK, and both effects were insensitive to pertussis toxin. Perfusion of an anti-G q/11α antibody into the pipette suppressed I K,BK, but not the inhibition of I Ca,V by BK. Thus, BK couples to I K,BK via G q/11, but coupling to I Ca,V is most likely via a different, pertussis toxin-insensitive G protein.
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