Brachychiton populneus (Schott & Endl.) R.Br. ameliorate carbon tetrachloride induced oxidative stress through regulation of endoplasmic reticulum stress markers and inflammatory mediators in Sprague-Dawley male rats

Abstract

In this study hepatoprotective aptitude of Brachychiton populneus against carbon tetrachloride (CCl4) instigated liver injuries in rats was investigated. High-performance liquid chromatography (HPLC) with a diode array detector (DAD) analysis of methanol extract of B. populneus (BPM) indicated existence of rutin, catechin and myricetin. Administration of CCl4 to rat decreased (p < 0.01) the level of catalase (CAT), total superoxide dismutase (SOD), peroxidase (POD), soluble protein and reduced glutathione (GSH) whereas elevated the concentration of H2O2, thiobarbituric acid reactive substances and nitrite in hepatic samples. In serum the level of hepatic markers; aspartate transaminase, alanine transaminase, alkaline phosphatase and total bilirubin increased with CCl4 treatment against control animals. In hepatic samples the expression level of endoplasmic reticulum stress associated genes like glucose regulated protein (GRP78), x-box binding protein- 1 total (XBP-1 t), x-box binding protein- 1 spliced (XBP-1 s), x-box binding protein- 1 unspliced (XBP-1 u), glutamate-cysteine ligase catalytic subunit (GCLC) and pro-inflammatory cytokines; tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and monocyte chemoattractant protein-1 (MCP-1) was elevated many fold with CCl4 administration to rat. Co-administration of BPM along with CCl4 to rats decreased (p < 0.05) the expression of above genes except GCLC where expression level was enhanced as compared to CCl4 treatment. Histopathology of liver showed injuries of hepatocytes, infiltration of leukocytes and damaged central lobule in CCl4 treated rats. However, BPM administration to CCl4 intoxicated rats restored the altered parameters towards the control rats. These results suggested the presence of antioxidant and anti-inflammatory constituents in methanol extract of B. populneus.