Abstract
An urgent need exists to identify more brown planthopper (Nilaparvata lugens Stål, BPH) resistance genes, which will allow the development of rice varieties with resistance to BPH to counteract the increased incidence of this pest species. Here, using bioinformatics and DNA sequencing approaches, we identified a novel BPH resistance gene, LOC_Os06g03240 (MSU LOCUS ID), from the rice variety Ptb33 in the interval between the markers RM19291 and RM8072 on the short arm of chromosome 6, where a gene for resistance to BPH was mapped by Jirapong Jairin et al. and renamed as “Bph32”. This gene encodes a unique short consensus repeat (SCR) domain protein. Sequence comparison revealed that the Bph32 gene shares 100% sequence identity with its allele in Oryza latifolia. The transgenic introgression of Bph32 into a susceptible rice variety significantly improved resistance to BPH. Expression analysis revealed that Bph32 was highly expressed in the leaf sheaths, where BPH primarily settles and feeds, at 2 and 24 h after BPH infestation, suggesting that Bph32 may inhibit feeding in BPH. Western blotting revealed the presence of Pph (Ptb33) and Tph (TN1) proteins using a Penta-His antibody, and both proteins were insoluble. This study provides information regarding a valuable gene for rice defence against insect pests.
Highlights
Provide insights into the molecular mechanisms of plant-insect interactions and serve as resources for molecular brown planthopper (BPH)-resistant rice breeding
The LOC_Os06g03240 (MSU LOCUS ID) encoding 194 amino acids was chosen because its protein contained a scop dig6xa_domain, which was described as a Kunitz/Bovine pancreatic trypsin inhibitor domain (Table S2a and Fig. 1a)
Later research confirmed that the sequence of bph[2] derived from ASD7 is completely identical to the sequence of BPH26 derived from ADR5211
Summary
Provide insights into the molecular mechanisms of plant-insect interactions and serve as resources for molecular BPH-resistant rice breeding. A dominant gene against BPH, Bph[32], was cloned from the rice variety Ptb[33] in an approximately 190-kb interval flanked by the markers RM19291 and RM8072 on the short arm of chromosome 6 using bioinformatics analysis and a transgenic approach. Bph[32] encodes a short consensus repeat (SCR) domain-containing protein that confers an antibiosis resistance to BPH and is localized in the plasma membrane of the cell. This gene is highly expressed in the leaf sheaths, where the BPH first settles and feeds. The results confirm that Bph[32] is a stable BPH resistance gene and provides a valuable gene for rice defence against insect pests
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