BPGQD/g-C3N4 nanocomposites as sensitive and selective platform for fluorescence and RGB based detection of vitamin B12

Abstract

Here, a brand-new fluorescence BPGQD/g-C3N4 nanocomposite for measuring Vitamin B12 has been created. The technique is based on the interaction between Vitamin B12 and graphitic nitride nanosheets modified with graphene quantum dots co-doped with boron and phosphorus. The developed nanocomposites have been characterized by using various techniques such as HR-TEM, SEM, EDS, XRD, FT-IR, XPS, Photoluminescence and UV–Vis absorption spectroscopy. Different excitation wavelengths were used, with a 320 nm excitation wavelength and a 416 nm emission wavelength chosen for fluorometric detection. It examined how several factors, including the sample solution's pH, the length of the incubation period, and the impact of NaCl concentration, affected the intensity of the fluorescence. A linear standardization curve was validated in the Vitamin B12 range of 0–60 nM under the authorized conditions. The limit of detection (LOD) and limit of quantitation (LOQ) were 9.61 nM and 29.12 nM respectively. Using newly created nanocomposites, an RGB-based sensor for Vitamin B12 detection was also constructed. Using this probe, it was possible to determine the amount of Vitamin B12 in human urine samples with respectable outcomes and satisfactory recoveries.