Abstract
Box–Behnken design-based HPLC optimization for quantitative analysis of chloramphenicol and hydrocortisone acetate in cream
Highlights
Chloramphenicol (CL), chemically D-(-)-threo-1-p-nitrolphenyl-2-dichloroacetamido 1,3-propanediol, is an antibiotic having a broad-spectrum antibacterial activity to treat either Gram-negative or Gram-positive bacterial infections and is widely used for the treatment of bacterial conjunctivitis
Inserting chloramphenicol (CL) and hydrocortisone acetate (HCA) in cream preparation is intended to have activity against skin infection and dermatitis and such a product is available in the Indonesian market
high-performance liquid chromatography (HPLC) method was validated according to the International Conference on Harmonization by determining several analytical performances intended for the method’s purpose
Summary
Chloramphenicol (CL), chemically D-(-)-threo-1-p-nitrolphenyl-2-dichloroacetamido 1,3-propanediol, is an antibiotic having a broad-spectrum antibacterial activity to treat either Gram-negative or Gram-positive bacterial infections and is widely used for the treatment of bacterial conjunctivitis. Khotimah et al / Journal of Applied Pharmaceutical Science 10 (09); 2020: 134-139 with densitometric measurement for the quantitative analysis of HCA using absorbance mode at 250 nm (Pyka et al, 2011), analysis of HCA and its related compounds (Dołowy et al, 2014) as well as simultaneous analysis of CL and HCA (Kristiningrum and Rakhmawati, 2012), high-performance liquid chromatography (HPLC) for quantitative analysis of HCA in antibiotics preparation (Bracey et al, 1966), analysis of CL and other drugs with a UV detector at 254 nm (Iqbal et al, 2006), and analysis of CL and HCA with UV detection at 259 nm (Katakam, 2014) Among these methods, HPLC is the most reported one due to its capability to provide the best separation of analytes and to get versatility in selecting the HPLC condition. The experimental Box–Behnken design (BBD) was used
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