Abstract
The effect of treating sheep red blood cells (SRBC) with neuraminidase or 2-amino-ethylisothiouronium bromide (AET) and different media (fetal bovine serum, Ficoll or dextran) during E rosetting of bovine lymphocytes was examined. It was found that the use of AET treated SRBC along with 6% dextran medium gave 60% and 83% E rosettes with bovine peripheral blood leukocytes and fetal thymocytes respectively. Furthermore, under these conditions the assay time was greatly reduced. Since these rosettes were shown to be specific markers of bovine T lymphocytes and such rosettes were stable over a 2 h period at 37°C the possible use of the present technique is discussed in regard to the isolation of purified T lymphocyte or T lymphocyte depleted populations which can subsequently be used for functional studies.
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