Abstract
Abstract: The objective of this work was to evaluate sperm cell motility after intrafollicular artificial insemination (IFAI) in vivo or after incubation in follicular fluid in vitro. In the in vivo experiment, IFAI was performed, followed by the recovery of follicular content 1 to 4 hours later, in order to assess sperm motility. In the in vitro experiment, spermatozoa from a pool of commercial frozen-thawed semen were evaluated for their kinetics after incubation for 1 or 3 hours, either pure (pool, control group) or in follicular fluid (FF). A low motility of sperm cells was observed in the FF samples, both in vitro and in vivo. In vitro, the main parameters negatively affected in the sperm cells incubated in FF, compared with the control, were: total motility (TM), progressive motility (PM), curvilinear distance, and straightness, after 1 hour of incubation; and TM, PM, average path velocity, and curvilinear velocity after 3 hours of incubation. The ovarian follicle and follicular fluid do not provide a suitable environment to maintain bovine sperm cell motility.
Highlights
Resumo – O objetivo deste trabalho foi avaliar a motilidade de espermatozoides após inseminação artificial intrafolicular (IAIF) in vivo ou após incubação em fluído folicular in vitro
Intrafollicular artificial insemination (IFAI) is the introduction of sperm cells inside a pre-ovulatory follicle through a transvaginal intrafollicular injection (Lucena et al, 1991). This assisted reproductive technique was first performed in women, as an alternative infertility treatment, when there was no response to in vitro fertilization (Lucena et al, 1991; Lucchini et al, 2012)
There is only one known report of IFAI increasing the pregnancy rate of cows with low fertility records due to heat stress (López-Gatius & Hunter, 2011). Since this previous study did not investigate the interaction between bovine sperm cells and follicular fluid (FF) after IFAI, the hypothesis that sperm deposition inside preovulatory follicles allows the maintenance of sperm cell viability still needs to be confirmed
Summary
Resumo – O objetivo deste trabalho foi avaliar a motilidade de espermatozoides após inseminação artificial intrafolicular (IAIF) in vivo ou após incubação em fluído folicular in vitro. Intrafollicular artificial insemination (IFAI) is the introduction of sperm cells inside a pre-ovulatory follicle through a transvaginal intrafollicular injection (Lucena et al, 1991). The objective of this work was to evaluate sperm cell motility after intrafollicular artificial insemination in vivo or after incubation in follicular fluid in vitro.
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