Bovine serum albumin nanoparticles as controlled release carrier for local drug delivery to the inner ear.

Zhan Yu,Ge Hong,Min Yu,Qingqing Xiong,Zhibao Zhang

doi:10.1186/1556-276x-9-343

Abstract

Nanoparticles have attracted increasing attention for local drug delivery to the inner ear recently. Bovine serum albumin (BSA) nanoparticles were prepared by desolvation method followed by glutaraldehyde fixation or heat denaturation. The nanoparticles were spherical in shape with an average diameter of 492 nm. The heat-denatured nanoparticles had good cytocompatibility. The nanoparticles could adhere on and penetrate through the round window membrane of guinea pigs. The nanoparticles were analyzed as drug carriers to investigate the loading capacity and release behaviors. Rhodamine B was used as a model drug in this paper. Rhodamine B-loaded nanoparticles showed a controlled release profile and could be deposited on the osseous spiral lamina. We considered that the bovine serum albumin nanoparticles may have potential applications in the field of local drug delivery in the treatment of inner ear disorders.

Highlights

Inner ear disorders, including sensorineural hearing loss (SSHL), commonly occur in clinics
We demonstrated that bovine serum albumin (BSA) can form nanospheres by desolvation method and can be used for local drug delivery
There was still obvious fluorescent signals with a larger area in the right ear (Figure 5b), which indicated that rhodamine B (RhB)-Bovine serum albumin (BSA)-NPs was retained nearby the round window membrane (RWM) and RhB possibly diffused into the Eustachian tube and the inner ear

Summary

Background

Inner ear disorders, including sensorineural hearing loss (SSHL), commonly occur in clinics. Tan et al demonstrated that brain-derived neurotrophic factor encapsulated in nanoporous poly(L-glutamic acid) particles could be released in a sustained manner with maintained biological activity and efficiently rescue primary auditory neurons in the cochlea of guinea pigs with sensorineural hearing loss [6]. Interest for the treatment of inner ear diseases for their drug loading and sustained release capacity Various methods such as desolvation, emulsion, template, microfluidic, mechanical stretching, and self-assembly have been reported to prepare protein-based particles or capsules [7,8,9,10,11]. The BSA-NPs would be prospectively considered as controlled release carriers for local drug delivery in the treatment of inner ear disorders

Methods

Conclusions

Findings

Schuknecht HF