Bovine Serum Albumin-Immobilized Black Phosphorus-Based γ-Fe2O3 Nanocomposites: A Promising Biocompatible Nanoplatform.

Atanu Naskar,Sohee Lee,Semi Kim,Dongjoon Ko,Kwang-Sun Kim

doi:10.3390/biomedicines9080858

Abstract

The interactions between proteins and nanoparticles need to be fully characterized as the immobilization of proteins onto various nanoplatforms in the physiological system often results in the change of surface of the protein molecules to avoid any detrimental issues related to their biomedical applications. Hence, in this article, the successful low-temperature synthesis of a BP-based γ-Fe2O3 (IB) nanocomposite and its interactive behavior with bovine serum albumin (BSA)—a molecule with chemical similarity and high sequence identity to human serum albumin—are described. To confirm the formation of γ-Fe2O3 and the IB nanocomposite, X-ray diffraction, transmission electron microscopy, and X-ray photoelectron spectroscopy analyses of the materials were performed. Additionally, the physical interaction between BSA and the IB nanocomposite was confirmed via UV–Vis and photoluminescence spectral analyses. Finally, the biocompatibility of the BSA-immobilized IB nanocomposite was verified using an in vitro cytotoxicity assay with HCT-15 colon cancer cells. Our findings demonstrate that this newly developed nanocomposite has potential utility as a biocompatible nanoplatform for various biomedical applications.

Highlights

IntroductionThe unique physicochemical properties of nanomaterials, such as their high surface area-to-volume ratio and specific size-dependent properties, have facilitated their use in drug delivery, bioimaging, antibacterial, biosensing, and other applications [3,4]
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Various polymeric and inorganic nanomaterial have been employed for biomedical applications such as gene silencing, gene therapy, drug delivery, gene delivery, bioimaging, and others [5,6,7,8,9,10,11,12,13]

Summary

Introduction

The unique physicochemical properties of nanomaterials, such as their high surface area-to-volume ratio and specific size-dependent properties, have facilitated their use in drug delivery, bioimaging, antibacterial, biosensing, and other applications [3,4]. In this regard, various polymeric and inorganic nanomaterial have been employed for biomedical applications such as gene silencing, gene therapy, drug delivery, gene delivery, bioimaging, and others [5,6,7,8,9,10,11,12,13]

Objectives

Methods

Conclusion