Bovine serum albumin-based nanoparticles containing resveratrol: Characterization and antioxidant activity

Abstract

In this study, bovine serum albumin (BSA) nanoparticles containing resveratrol (RVT) were developed to improve the biopharmaceutical characteristics of the RVT. BSA nanoparticles containing RVT (RVT-NP) were obtained by the coacervation technique and presented mean diameter of 175 ± 6 nm, polydispersity of 0.130 ± 0.01, zeta potential of −37.4 ± 1.3 mV and encapsulation efficiency about 60%. Morphological analysis revealed spherical and smooth nanoparticles. In vitro release profile was prolonged and after 144 h about 26% of RVT were released. The release kinetic followed the second order model, characterized by a rapid initial release followed by a slower RVT release diffusion-controlled. Nanoencapsulation process resulted in RVT amorphization and chemical interactions between RVT and BSA. Nanoparticles were stable over 90 days at room temperature and under refrigeration (2–8 °C). In the hypochlorous acid scavenging activity, RVT-NP presented inferior activity than free RVT, probably due to the prolonged RVT release. Blank BSA nanoparticles also presented scavenging activity, thus, antioxidant activity of RVT-NP was an addition of the effects of the RVT and BSA. Thus, BSA nanoparticles are promising carriers for prolonged RVT release and application in oxidative stress-based diseases.