Abstract
Materials and methods The study was conducted in 1998-1999. It included 18 cattle herds which had problems with bovine respiratory tract disease complex. Four to five diseased calves from each farm were chosen for closer examination. A thorough clinical examination and tracheobronchial lavage were done. Blood samples were taken from the calves at the acute phase and 3-4 weeks later. In addition, 10 blood samples from cattle of different age were taken from the same herds. Because bovine herpesvirus-1 (BHV-1) does not exist in Finland and also bovine virus diarrhoea virus (BVDV) is very rare, serum samples were tested for antibodies to bovine parainfluenza virus3 (PIV-3), bovine respiratory syncytial virus (BRSV), bovine coronavirus (BCV), bovine adenovirus-3 (BAV-3) and bovine adenovirus-7 (BAV-7). Some samples were also tested for antibodies to BVDV. ELISA-test was used for antibodies against PIV-3, BRSV, BCV and BVDV. Virus neutralization test was used for BAV-3 and BAV-7. Bacteria were cultured from lavage fluid and in vitro susceptibility for selected antimicrobials (ampicillin, penicillin, trimethoprim-sulfamethoxazole, gentamicin, tetracycline and ciprofloxacin) tested. One calf was autopsied.
Highlights
Antibodies to parainfluenza virus3 (PIV-3) were found in all 18 herds (100%)
Because bovine herpesvirus-1 (BHV-1) does not exist in Finland and bovine virus diarrhoea virus (BVDV) is very rare, serum samples were tested for antibodies to bovine parainfluenza virus3 (PIV-3), bovine respiratory syncytial virus (BRSV), bovine coronavirus (BCV), bovine adenovirus-3 (BAV-3) and bovine adenovirus-7 (BAV-7)
Antibodies to BCV were discovered from 16/18 herds (89%)
Summary
Antibodies to PIV-3 were found in all 18 herds (100%). Antibodies to BCV were discovered from 16/18 herds (89%). Antibodies to BRSV were found from 12/18 herds (67%) but these positive findings were all individual animals. Antibodies to BAV-3 and BAV-7 were observed from 15/18 farms (83%) and 18/18 farms (100%) respectively. Antibodies to BVDV were not detected at all (0/6). Bacterial growth was found in 19 samples (22%) at 10 herds (56%). The most common finding was Pasteurella sp., which was monitored from 14 samples (74% of positive samples) at 8 herds (44%). Strains were in vitro susceptible to all antimicrobials tested. All 18 herds had mycoplasma findings; Mycoplasma dispar was found from 17 herds (94%) and Mycoplasma sp. All colonies that were examined using immunofluorescence test proved to be M. bovirhinis colonie
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