Abstract
Bovine papillomavirus (BPV) is an oncogenic virus related to serious livestock diseases. Oncoproteins encoded by BPV are involved in several steps of cellular transformation and have been reported as presenting clastogenic effects in peripheral lymphocytes and primary culture cells. The aim of this study was to evaluate the clastogenic potential of BPV types 1, 2, and 4 by comet assay. Peripheral blood was collected from 37 bovines, 32 infected with different levels of papillomatosis (12 animals have no affection) and five calves, virus free (negative control). The viral identification showed presence of more than one virus type in 59.375% of the infected animals. Comet assay was performed according to alkaline technique. The Kruskal-Wallis test showed statistical difference between the negative control group and infected animals (P = 0.0015). The Dunn post hoc test showed difference comparing the infected animals with calves. Mann-Whitney U test verified no difference between animals infected with only one viral type and animals presenting more than one viral type. The comet assay is considered an efficient tool for assessment of damage in the host chromatin due to viral action, specifically highlighting viral activity in blood cells.
Highlights
Bovine papillomavirus (BPV) is a widespread oncogenic virus found worldwide belonging to the Papillomaviridae family, which displays tropism for squamous epithelial and mucosal tissues
We selected the specific primers for BPV-1, -2, and -4 due to their prevalence in the herd, and we could detect the virus sequences in peripheral blood cells collected from the adult animals, with and without skin papillomas (Figure 1(a))
The carcinogenic mechanisms related to BPV are not yet fully elucidated; it is known that the malignancy is caused type, the frequency of nucleoids observed per class, and the comet score. (b) Kruskal-Wallis statistic to compare the clastogenicity among calves, asymptomatic and symptomatic cattle, from the comet score. (c) Comparison between comet score observed in monoinfected and coinfected bovines through Mann-Whitney U test
Summary
Bovine papillomavirus (BPV) is a widespread oncogenic virus found worldwide belonging to the Papillomaviridae family, which displays tropism for squamous epithelial and mucosal tissues. These viruses are associated with benign and malignant epithelial lesions. The papillomavirus genome is divided into three regions: early, late, and noncoding long control region (LCR), separated by two polyadenylation sites [3]. The early control region occupies 50% of the viral genome and encodes E1, E2, E3, E4, E5, E6, and E7 proteins. The late control region occupies 40% of the genome and contains the genes that codify L1 and L2 capsid proteins and LCR, which comprises
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
