Abstract
Rapid equilibrium dialysis has been used to show that recombinant bovine brain inositol monophosphatase binds one equivalent of P i per subunit of enzyme. P i is only bound in the presence of Mg 2+ ions. The dissociation constant for the equilibrium is approximately 50 μM. This value of K d is independent of the concentration of the Mg 2+ ions and of the presence or absence of Li + ions. Lithium ions which inhibit the enzyme uncompetitively are not able to support the binding of the P i to the enzyme. The observation that P i only binds in the presence of Mg 2+ ions supports similar conclusions made in experiments which studied the protection of the enzyme from proteolytic degradation and chemical modification.
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