Abstract
The authors assessed the possibility of substitution of the serum component with tissue extracts (muscles, liver, kidneys) of bovine fetuses in the culture medium during the cultivation of transplanted LEK and Vero cell lines, as well as the reproduction of infectious rhinotracheitis IR, PI-3 viruses and reovirus on them. The greatest stimulating effect on LEK and Vero cells was obtained from bovine fetuses muscle extract. The effect of this extract on the proliferative activity of LEK and Vero cells is significant and amounts to 27 and 25%, respectively. The power of the effect of liver and kidney extracts is significantly lower and equal, respectively, 15 and 18% for LEK and 14 and 19% for Vero, although it is reliable. The reproductive activity of IR and PI-3 viruses when using tissue extracts was inferior to that when using blood serum. The stimulating effect of blood serum and muscle extract on the reproduction of reovirus was comparable. The effect of fetal muscle extract on the reproduction of IR, PI-3 viruses and reovirus is reliable and amounts to 29, 31 and 33%, respectively. In general, it is close to that of the blood serum of bovine fetuses - 30, 35 and 36%. The power of the influence of the liver and kidney extracts of the bovine fetuses is significantly lower and comparable to that of the blood serum of the cows themselves: 25, 23 and 20%, although it is reliable.
Highlights
Today the most important component of the nutrient medium for the production of somatic eukaryotic cells is the blood serum of animals
The greatest proliferation was provided by muscle extract, it was inferior in this bovine serum
Despite the low content of total protein in tissue extracts of cow fruits in comparison with bovine blood serum of [18], about 50% of it in the reserach extracts is represented by albumin, while in serum their share does not exceed 29%
Summary
Today the most important component of the nutrient medium for the production of somatic eukaryotic cells is the blood serum of animals. It remained an indispensable component for the growth media of most cell cultures. It remains an irreplaceable component of the growth medium for most cell cultures [1]. The use of animal blood serum has a number of significant limitations. It is quite expensive, especially if it is obtained from animal fenses. When cells are produced for the purpose of viral reproduction, blood serum can inhibit this process due to the possible presence of antibodies to the virus being produced and increase the risk of contamination, for example, with other viruses
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