Bovine dentin collagen/poly(lactic acid) scaffolds for teeth tissue regeneration

Abstract

Electrospun scaffolds with diameter fibers compared to those in the extracellular matrix were produced with poly(lactic acid) (PLA) and non-denatured collagen from bovine dentin (DCol). DCol was obtained through an improved version of the Longin method by acid erosion of the hydroxyapatite of the roots of teeth from a 2-year-old cattle. The dentin collagen was characterized by energy dispersive X-ray spectroscopy (EDS), and carbon, nitrogen, and oxygen were found to be the main elements of the protein. Infrared analysis revealed the typical bands of collagen at about 3300, 1631, 1539, and 1234 cm−1 for amides A, I, II, and III, respectively. Calorimetric and infrared analyses also demonstrated that the collagen was non-denatured. With scanning electron microscopy, it was found that the thinnest fibers with a diameter comparable to that of fibers in the extracellular matrix were obtained when dentin collagen and acetic acid (AAc) were added to the solution of PLA in trifluoroethanol (TFE). The scaffolds with the thinnest diameter had also the highest porosity, and we considered that they could be beneficial in the growth of dentin cell. Human placenta-derived mesenchymal stem cells were seeded onto electrospun scaffolds. After 24, 48 and 96 h of culture, cell proliferation was evaluated by two independent strategies. In both assays, it was found that the pl-MSCs were capable of adhering and proliferating in different scaffolds. It was also observed that cell adhesion and proliferation increased significantly in scaffolds containing collagen, although the addition of AAc slightly decreased this effect on all scaffolds.Graphical abstract