Abstract

Bottom-up synthetic biology aims at reconstructing a cell from biomolecular constituents. However, the combination of multiple elements and functions remained elusive, which stimulates endeavors to explore entirely synthetic bio-inspired solutions towards engineering life. To this end, DNA nanotechnology represents one of the most promising routes, given the inherent sequence specificity, addressability, and programmability of DNA. Here, we demonstrate functional DNA-based cytoskeletons operating in microfluidic cell-sized compartments and lipid vesicles.

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