Abstract
The HO gene, which encodes an endonuclease responsible for initiating mating type switching in yeast, is transcribed at START during the cell cycle of mother cells but not at all during the cell cycle of daughter cells. At least six genes, called SWI1–6, are necessary for HO transcription. We describe the isolation and characterization of mutations in two new genes called SDI1 and SDI2, which partially suppress the requirement for SWI5 and which cause daughter cells to express HO. The analysis of mating type switching in swi5 − sdi1 − and SWI5 + sdi1 − strains suggests that the mother cell specificity of HO transcription is due exclusively to the selective action of SWI5 in mother cells. SDI1 encodes (or regulates) a repressor protein that binds to the HO promoter and prevents HO transcription in daughter cells by causing HO to be fully SWI5 dependent.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
