Abstract
BackgroundBostrycin is a novel compound isolated from marine fungi that inhibits proliferation of many cancer cells. However, the inhibitory effect of bostrycin on lung cancers has not been reported. This study is to investigate the inhibitory effects and mechanism of bostrycin on human lung cancer cells in vitro.MethodsWe used MTT assay, flow cytometry, microarray, real time PCR, and Western blotting to detect the effect of bostrycin on A549 human pulmonary adenocarcinoma cells.ResultsWe showed a significant inhibition of cell proliferation and induction of apoptosis in bostrycin-treated lung adenocarcinoma cells. Bostrycin treatment caused cell cycle arrest in the G0/G1 phase. We also found the upregulation of microRNA-638 and microRNA-923 in bostrycin-treated cells. further, we found the downregulation of p110α and p-Akt/PKB proteins and increased activity of p27 protein after bostrycin treatment in A549 cells.ConclusionsOur study indicated that bostrycin had a significant inhibitory effect on proliferation of A549 cells. It is possible that upregulation of microRNA-638 and microRNA-923 and downregulaton of the PI3K/AKT pathway proteins played a role in induction of cell cycle arrest and apoptosis in bostrycin-treated cells.
Highlights
Bostrycin is a novel compound isolated from marine fungi that inhibits proliferation of many cancer cells
Bostrycin inhibited the proliferation of A549 cells First, we used the MTT assay to detect effect of bostrycin on A549 cell proliferation
There was a dose-dependent and time-dependent inhibition of A549 cell proliferation by bostrycin (Figure 1) with an optimal linear relationship seen between 10-30 μΜ of bostrycin
Summary
Bostrycin is a novel compound isolated from marine fungi that inhibits proliferation of many cancer cells. The inhibitory effect of bostrycin on lung cancers has not been reported. This study is to investigate the inhibitory effects and mechanism of bostrycin on human lung cancer cells in vitro. Despite recent advances in surgical techniques and chemotherapy/radiotherapy strategies, the long-term survival rates remain poor. Growth factor signaling pathways have been shown to be important targets in lung cancer therapy. Targeting such intracellular pathways that regulate proliferation, apoptosis, metastasis and resistance to chemotherapy represents an important therapeutic strategy for lung cancer [1]. Marine microorganisms can grow under adverse conditions such as low temperatures, high pressures, and poor nutrition. The diversity of biological activities in these environments exceeds those of land organisms
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