Boron-resistant Alternaria alternata (OG14) mitigates boron stress by improving physiological and antioxidative response in wheat (Triticum aestivum L.)

Abstract

Effect of Alternaria alternata (OG14) isolated from a rock lichen (Xanthoria sp.) was investigated on the relief of boron stress in wheat. To determine the tolerance level to B stress, the fungus was grown at increasing boric acid (BA) concentrations in the range of 0.0–2.5 M. No significant change in colony development of the fungus was observed up to 1 M BA application compared to the control but after this dose, it decreased depending on the increase in the BA dose. When the element content of wheat seedlings was evaluated by ICP-MS, BA application increased B content together with Mg, P, K, Fe contents of the seedlings to very high levels compared to the control. However, fungus + BA treatments decreased the content of B and the other elements in the seedlings. The BA applications resulted in an increase in the levels of reactive oxygen species, including H2O2 and O2.−as well as lipid peroxidation in the seedlings. However, when the fungal inoculation was performed under the same BA conditions, the levels of these parameters decreased. The fungus inoculation stimulated the activity of all studied enzymes compared to BA applications. BA applications alone increased non - enyzmatic the oxidized ascorbate level more than the reducing ascorbate, leading to a decrease in the AsA/DHA ratio. The results show that A. alternata treatment can mitigate the negative effects of B stress on wheat seedlings by reducing ROS, LPO, B content, increasing the capacity of enzymatic and non-enzymatic antioxidants, and improving root and shoot length.