Abstract
Brassinosteroids (BRs) are natural, nontoxic, non-hazardous, biosafe, and eco-friendly plant hormones, possessing diverse pharmacological activities. However, little is known about the type and content of BRs in frequently consumed plant-derived foodstuffs because of their low abundance and high abundance of interference. In this study, a selective, accurate, and sensitive method based on the online solid-phase extraction using the boronic acid-functionalized Scholl-coupling microporous polymer was developed for the analysis of BRs in plant-derived foodstuffs. Under optimum conditions, an excellent linearity (R2 ≥ 0.9970) and lower limits of detection (0.010-0.070 pg mL-1) were obtained. The high relative recoveries were in the range of 90.33-109.34% with relative standard deviations less than 9.73%. The method was successfully used for the determination of BRs in fifteen plant-derived foodstuffs. The present work offers a valuable tool for exploring BRs from the plant-derived foodstuffs and can provide useful information for developing functional foods.
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