Abstract
Bordetella pertussis attachment to host cells is a crucial step in colonization. In this study, we investigated the specificity of antibodies, induced either by vaccination or infection, capable of reducing bacterial adherence to respiratory epithelial cells. Both sera and purified anti-B. pertussis IgG or IgA fractions efficiently reduced attachment. This effect was found to be mediated mainly by fimbriae-specific antibodies. Antibodies with other specificities did not significantly interfere in the interaction of B. pertussis with respiratory epithelial cells, with the exception of antifilamentous hemaglutinin antibodies, which reduced bacterial attachment. However, this effect was smaller in magnitude than that observed in the presence of fimbriae-specific antibodies. The strong agglutinating activity of antifimbriae antibodies seems to be involved in this phenomenon.
Highlights
The relative contribution of humoral and cellular immunity on host protection against B. pertussis is still underBordetella pertussis is the etiologic agent of whooping cough, a disease that is re-emerging in many parts of the world despite high vaccination coverage
The A549 cell line has previously been used to investigate the involvement of B. pertussis virulence factors on bacterial interaction with human respiratory epithelial cells (Schipper etal., 1994; Alonso et al, 2001; Ishibashi etal., 2001; Coutte et al, 2003)
Bordetella pertussis is an obligate human pathogen that resides in the respiratory tract during infection
Summary
The relative contribution of humoral and cellular immunity on host protection against B. pertussis is still under. Bordetella pertussis is the etiologic agent of whooping cough, a disease that is re-emerging in many parts of the world despite high vaccination coverage. The side effects of wholecell pertussis vaccines have prompted the development of acellular vaccines containing purified B. pertussis virulence factors. Detailed insight into mechanisms protective against B. pertussis should facilitate a more rational selection of vaccine components. Bordetella pertussis is an obligate human pathogen infecting the respiratory tract. The interaction between B. pertussis and the host involves several virulence factors. Surface-associated proteins such as fimbriae (Fim), filamentous hemaglutinin (FHA) and pertactin (Prn), have all been implicated in bacterial attachment to host cells, whereas toxins such as pertussis toxin (Ptx), adenylate cyclase toxin (Cya), lipopolysaccharide (LPS), among others, are regarded as being responsible for pertussis symptoms (Hewlett, 1997; Locht, 1999)
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
