Abstract
Advancements in shoot regeneration systems support biotechnology-based tools used in the genetic improvement of plant crops. This study aims to enhance shoot regeneration in potatoes by boosting polyamine content by adding AgNO3 to the shoot regeneration medium (MS medium supplemented with 30 g L−1 sucrose, 100 mg L−1 myoinositol, and 2.25 BA mg L−1). Five concentrations of AgNO3 (2, 4, 6, 8, and 10 mg L−1) were used in addition to a control. The effect of AgNO3 on regeneration assumed a more or less concentration-dependent bell-shaped curve peaking at 4 mg L−1. Enhancements in shoot regeneration were attributed to the known role of AgNO3 as an ethylene action blocker in addition to improvements in polyamine accumulation without an increase in H2O2 content, lipid peroxidation, or DNA damage. The uncoupling of shoot regeneration and polyamine content recorded at high AgNO3 concentrations can be attributed to the consumption of polyamines to counteract the synchronized oxidative stress manifested by increases in H2O2 content, lipid peroxidation, and DNA damage.
Highlights
Potato (Solanum tuberosum) is the most important non-cereal food crop with annual world production of 370 million tons harvested from 17.34 million hectares (FAO 2019 https://www.fao.org/faostat/ar/#data/QCL, accessed on 11 November 2021)
Tubers were stored at 10 ◦ C till sprouting, sprouts were detached from tubers and submerged in tap water containing a few drops of a liquid detergent, shaken for 10 min, rinsed in running tap water for 30 min to remove the detergent
On the callus induction medium, the explants swelled, and nodular green calli started to appear at the cut margins of all internode explants after 2 weeks (Figure 2)
Summary
Potato (Solanum tuberosum) is the most important non-cereal food crop with annual world production of 370 million tons harvested from 17.34 million hectares (FAO 2019 https://www.fao.org/faostat/ar/#data/QCL, accessed on 11 November 2021). Tubers are an essential origin for energy and protein. They introduce essential nutrients to a diet, including vitamins (B1 , B3 , B6 and C), minerals (potassium, phosphorus, magnesium, zinc, and iron) in addition to folate, pantothenic acid, riboflavin, and dietary fiber [1]. Compared to other food crops, potatoes can produce more energy, proteins, minerals, and vitamins per unit of time and land area [2]. Conventional potato breeding is a difficult task due to the tetraploid nature and high heterozygosity of the potato genomes. The first is responsible for inbreeding depression and intra-species incompatibilities, while the second prevents the incorporation of new traits through conventional breeding [5]. Searching for functional genetic variability in wild relatives is laborious and time-consuming [6,7]
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