Abstract
Morpholine derivatives expand the applicability of photobiocatalysis towards stabilization of flavin-based bio- and photocatalysts.
Highlights
NAD(P)H cofactors, is the use of light in the presence of a sacrificial electron donor (ED).[4]
We have shown that the MOPS buffer acts as an ED in photobiocatalytic systems and enhances the stability of the cyclohexanone monooxygenase from Acinetobacter calcoaceticus (CHMOAcineto).[22]
Effect of morpholines on enzymatic photoreduction First, we investigated the effect of MOPS on the photobiocatalytic reduction of ketoisophorone (1a) to levodione (1b) by using either TsOYE, the old yellow enzyme homologue from Thermus scotoductus SA-01, or XenB, an enoate reductase from Pseudomonas sp.,[23] using FMN as both an enzyme cofactor and a photocatalyst (Fig. 1)
Summary
NAD(P)H cofactors, is the use of light in the presence of a sacrificial electron donor (ED).[4]. Stability measurements were performed by incubating 10 μM enzyme at 30 °C in 100 mM Tris-HCl or 100 mM MOPS, pH 7.5, containing 100 μM FMN.
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