Boosting laccase activity by synergistic drive of near infrared laser, surfactant, and sodium chloride for healthcare coatings with UV resistance, anti-oxidation, and photothermal conversion

Abstract

Natural enzymes as green and efficient biocatalysts are essential in constructing multifunctional organic coatings for meeting the green chemistry processes. Herein, the laccase activity was for the first time enhanced by synergistic drive of near infrared (NIR) laser, dodecyldimethyl (3-sulfopropyl) ammonium hydroxide internal salt (SDDAB), and sodium chloride (NaCl). The SDDAB activation on laccase activity is unpredictable, which is advanced compared with conventional predictable ones, including adding mediator and improving reactive temperature. The NaCl can again boost the laccase activity by 29.8 % in the presence of SDDAB. Moreover, NIR laser irradiation again promotes laccase activity by 16.3 % in laccase/SDDAB/NaCl. The total specific activity of laccase exhibits an improvement of 29.2 %, indicating an advancement compared with the activities decrease in immobilized and encapsulated laccases. The Vm of laccase has been improved to 150.9 %. Besides, the hydrogen bonds and van der Waals force between laccase and SDDAB have been verified. Thus, laccase/SDDAB/NaCl was adopted to catalyze the polymerization of gallic acid to create eco-friendly UV-resistant, antioxidative, and photothermal coatings for healthcare. The ultraviolet protection factor (UPF), free radical scavenging ability, and photothermal temperature are 126.28, 95.0 %, and 94.2 °C, respectively. The multifunctional coating not only exhibits the response of sunlight-like photothermal conversion, but also possesses the photothermal response mediated by NIR laser. With the intensity increase of NIR laser irradiation, the surface temperature of modified cotton fabric is gradually strengthened from 29.5 to 43.8 °C. The surface characteristic and chemical composition of the multifunctional coatings were investigated by modern technology. The smartphone was adopted to quickly detect the red, green, and blue (RGB) values for demonstrating the successful fabricating of composite coatings, compared with traditional K/S characterization. Briefly, this work provided a novel, feasible, and elegant idea to boost laccase activity, inspiring more scholars to create advanced organic coatings with natural enzyme as the high-performance green catalysts for meeting green chemistry processes.