Boosting electrocatalytic activity of carbon fiber@fusiform-like copper-nickel LDHs: Sensing of nitrate as biomarker for NOB detection

Abstract

Morphological evolution of layered double hydroxides (LDHs) with preferential crystal facets has appealed gigantic attention of research community. Herein, we prepare hierarchical hybrid material by structurally integrating fusiform-like CuNiAl LDHs petals on conductive backbone of CF (CF@CuNiAl LDHs) and investigate electrocatalytic behavior in nitrate reduction over a potential window of ˗0.7 V to +0.7 V. The CF@CuNiAl LDHs electrode exhibits remarkable electrocatalytic aptitude in nitrate sensing including broad linear ranges of 5 nM to 40 µM and 75 µM to 2.4 mM with lowest detection limit of 0.02 nM (S/N = 3). The sensor shows sensitivity of 830.5 ± 1.84 µA mM1- cm2- and response time within 3 s. Owing to synergistic collaboration of improved electron transfer kinetics, specific fusiform-like morphology, presence of more catalytically active {111} facets and superb catalytic activity of LDHs, CF@CuNiAl LDHs electrode has outperformed as electrochemical sensor. Encouraged from incredible performance, CF@CuNiAl LDHs flexible electrode has been applied in real-time in-vitro detection of nitrite oxidizing bacteria (NOB) through the sensing of nitrate because NOB convert nitrite into nitrate by characteristic metabolic process to obtain their energy. Further, CF@CuNiAl LDHs based sensing podium has also been employed in in-vitro detection of nitrates from mineral water, tap water and Pepsi drink.