Bone regeneration with adipose derived stem cells in a rabbit model.

Michele Maglione,Mauro Melato,Enrico Salvador,Lorenzo Bevilacqua,Maria E Ruaro,Giuliana Tromba,Daniele Angerame

doi:10.7555/jbr.32.20160066

Abstract

It has been shown that stem cells are able to calcify both in vitro and in vivo once implanted under the skin, if conveniently differentiated. Nowadays, however, a study on their efficiency in osseous regeneration does not exist in scientific literature and this very task is the real aim of the present experimentation. Five different defects of 6 mm in diameter and 2 mm in depth were created in the calvaria of 8 white New Zealand rabbits. Four defects were regenerated using 2 different conveniently modified scaffolds (Bio-Oss® Block and Bio-Oss Collagen®, Geistlich), with and without the aid of stem cells. After the insertion, the part was covered with a collagen membrane fixed by 5 modified titan pins (Altapin®). The defect in the front was left empty on purpose as an internal control to each animal. Two animals were sacrificed respectively after 2, 4, 6, 10 weeks. The samples were evaluated with micro-CT and histological analysis. Micro-CT analysis revealed that the quantity of new bone for samples with Bio-Oss® Block and stem cells was higher than for samples with Bio-Oss® Block alone. Histological analysis showed that regeneration occurred in an optimal way in every sample treated with scaffolds. The findings indicated that the use of adult stem cells combined with scaffolds accelerated some steps in normal osseous regeneration.

Highlights

The repair of bone defects continues to be a challenging part of many reconstructive procedures[1–2]
Several recent studies have reported the ubiquitous distribution of adult stem cells in various tissues and organs, including bone marrow, muscle, brain, skin, and CLC number: R336, Document code: A The authors reported no conflict of interests
During histological analysis of the samples, we focused on the following fundamental aspects:

Summary

Introduction

The repair of bone defects continues to be a challenging part of many reconstructive procedures[1–2]. Several recent studies have reported the ubiquitous distribution of adult stem cells in various tissues and organs, including bone marrow, muscle, brain, skin, and CLC number: R336, Document code: A The authors reported no conflict of interests.

Objectives

Methods

Results

Conclusion