Bone protective effects and mechanism of Polygonatum Sibiricum Polysaccharide in ovariectomized rat

Abstract

Objective: To assess the bone protective effects and mechanism of Polygonatum Sibiricum Polysaccharide (PSP) in ovariectomized rat. Methods: 60 female SD rats were randomly separated into 6 groups:control group (n=10); ovariectomized group (OVX) (n=10); the OVX + E 2 group(OVX + E 2 ) (n=10); the high-dose OVX + PSP group (OVX + HZ) (n=10); the moderate-dose OVX + PSP group (OVX + MZ) (n=10); the low dose OVX + PSP group (OVX + LZ) (n=10). PSP was administered intragastrically to the rats. After 35 days, the total body Bone mineral density (BMD) was assessed in the rats. All sections were processed for hematoxylin-eosin staining (H.E.), and Basic fibroblast growth factor (bFGF), Tartrate-resistant acid phosphatase (TRAP), Bone morphogenetic proteins (BMP), and Bone Gla protein (BGP) immunoreactivity was assessed. Serum tumor necrosis factor-α (TNF-α) and bone specific alkaline phosphatase (BALP) levels were assessed using commercially available ELISA kits. Results:The expression of BMP and bFGF were significantly increased in OVX+HP. The expression of TRAP, TNF-α, BGP and BALP were significantly decreased in OVX+HP. BMD was significantly increased in OVX+HP(vs. OVX, P<0.05).Conclusion: The results of this experiment demonstrate that PSP can increase the expression of bFGFand BMP while reducing the expression of TRAP, BGP, TNF-α and BALP. The administration of PSP to ovariectomized rats reverses bone loss and prevents osteoporosis.