Bone marrow stromal antigen 2 , a novel ELL-associated factor 2 target gene, regulates proliferation and migration abilities of prostate cancer cells

Abstract

Objective To investigate whether bone marrow stromal antigen 2 (BST2) which was regulated by ELL-associated factor 2 (EAF2) can promote prostate cancer cells proliferation and migration. Methods After inhibiting the expression of EAF2 by 100 pmol interfering RNA (RNAi) in C4-2 cells using lipofectamine 2000, the mRNA and protein expression levels were assessed by real-time quantitative polymerase chain reaction (Real-time PCR) and Western blotting respectively. After transfecting MYC-EAF2 into 293T cells according to the manufacturer’s instruction, the expression level of BST2 was detected by Western blotting. After knockdown of EAF2 by RNAi in C4-2 cells, proliferation was tested by bromodeoxyuridine incorporation (BrdU) assay, and migration was tested by Transwell assay. Results The mRNA and protein levels were significantly increased after transfection of small interfering RNA (siRNA) against EAF2. Moreover, proliferation rate was further decreased from (22.09±1.08)% to (12.25±1.32)% (P=0.001). At the meantime, the number of migrating cells was decreased from (50.50±3.80)% to (24.25±1.43)% (P=0.001). Conclusion Our findings suggest that EAF2 regulates the mRNA and protein levels of BST2, and BST2 could regulate proliferation and migration abilities of C4-2 cells. Key words: Prostate cancer; ELL-associated factor 2; Bone marrow stromal antigen 2