Abstract
Irradiated mice of the (C3H × C57BL 10 )F 1 and C57BL 6 × DBA 2 )F 1 strains were reconstituted with an excess of syngeneic thymocytes containing antigen-reactive cells, and with graded limiting numbers of bone marrow cells containing precursors of antibody-forming cells (P-AFC), and then injected with sheep erythrocytes. The number of P-AFC and their possible specialization for serological properties of antibody were investigated by determining the titer of 2-mercaptoethanol-sensitive serum hemagglutinins and hemolysins 11 days after grafting. The limiting dilution assays gave different results in the two mouse strains, but clearly indicated that the number of detectable P-AFC per unit number of bone marrow cells was of the same order of magnitude for both antibody responses. However, agglutinins and lysins were not associated in a significant number of recipient mice receiving an average of 1 P-AFC. Hence, marrow precursor cells were restricted for serological properties of antibody reflecting structural features not equivalent to those underlying molecular class or subclass. Circumstantial evidence suggested that a subpopulation of marrow P-AFC characterized by larger burst size was detected by serum titrations, whereas more numerous P-AFC with smaller burst size were previously detected by enumeration of splenic plaque-forming cells.
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