Abstract
Introduction Hematopoietic cell transplantation (HCT) is a potentially curative treatment in myelofibrosis (MF). Nevertheless, in at least one-third of patients, a reliable parameter for disease response is lacking due to the absence of a molecular marker (in triple-negative MF) or the suboptimal sensitivity for CALR and MPL monitoring with routinely used PCR methods. Trephine biopsy provides a limited histological description, as it does not represent the fibrosis and/or blast eradication status of the entire bone marrow. The aim of this study is to identify magnetic resonance imaging (MRI) biomarkers that reflect response to transplant and may serve as predictors of transplant efficacy. Methods We report the case of a 45-year-old man diagnosed with triple-negative MF who was eligible for HCT following progression after a previous procedure. A pre-transplant trephine biopsy showed grade III fibrosis, according to the WHO 2016 classification. CD34+ cells were found to comprise 10%-15% of cells. He underwent MRI 8 days before bone marrow transplantation from a haploidentical donor. A second, comparative MRI was performed 6 months after HCT; concurrent chimerism was estimated through Short Tandem Repeats (STRs) analysis. A multiparametric protocol was applied, including intravoxel incoherent motion (IVIM) diffusion-weighted imaging, Dixon GRE with fat fraction calculation, and Turbo Spin Echo T1 and STIR sequences. MRI examination was performed on a 3 Tesla MRI machine and included lumbar segments L4 and L5, pelvis, sacral bone, and coccyx. A phased-array surface coil was applied to optimize signal-to-noise ratio. Finally, radiomics and texture analysis were performed on ADC sequences using specific image software post-analysis (Slicer 3D v.4.10.2). Results The selected MRI parameters showed substantial modifications between the first and second exams. T1 and STIR sequences demonstrated a decrease in bone marrow fibrosis (Figure 1). ADC values increased at pre-specified target points (iliac crest, pre-transplant: 0.762, post-transplant: 0.901; femur, pre-transplant: 0.77, post-transplant: 0.808). Moreover, among 300 features analyzed (Figure 2), 20 presented changes potentially related with bone marrow restoration. Concomitant STRs analysis showed a full donor chimerism status. Conclusions To the best of our knowledge, this is the first case of functional, textural analysis-based MRI employed in transplanted MF. Multiparametric MRI data, including anatomical and functional sequences (particularly IVIM with texture analysis of ADC values and Dixon with fat fraction calculation) may represent valuable biomarkers for accurate detection of bone marrow engraftment and chimerism. Disclosures No relevant conflicts of interest to declare.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.