Abstract
Gastric aspiration lung injury is one of the most common clinical events. This study investigated the effects of bone‐marrow‐derived mesenchymal stem cells (BMSCs) on combined acid plus small non‐acidified particle (CASP)‐induced aspiration lung injury. Enhanced green fluorescent protein (EGFP +) or EGFP − BMSCs or 15d‐PGJ 2 were injected via the tail vein into rats immediately after CASP‐induced aspiration lung injury. Pathological changes in lung tissues, blood gas analysis, the wet/dry weight ratio (W/D) of the lung, levels of total proteins and number of total cells and neutrophils in bronchoalveolar lavage fluid (BALF) were determined. The cytokine levels were measured using ELISA. Protein expression was determined by Western blot. Bone‐marrow‐derived mesenchymal stem cells treatment significantly reduced alveolar oedema, exudation and lung inflammation; increased the arterial partial pressure of oxygen; and decreased the W/D of the lung, the levels of total proteins and the number of total cells and neutrophils in BALF in the rats with CASP‐induced lung injury. Bone‐marrow‐derived mesenchymal stem cells treatment decreased the levels of tumour necrosis factor‐α and Cytokine‐induced neutrophil chemoattractant (CINC)‐1 and the expression of p‐p65 and increased the levels of interleukin‐10 and 15d‐PGJ 2 and the expression of peroxisome proliferator‐activated receptor (PPAR)‐γ in the lung tissue in CASP‐induced rats. Tumour necrosis factor‐α stimulated BMSCs to secrete 15d‐PGJ 2. A tracking experiment showed that EGFP + BMSCs were able to migrate to local lung tissues. Treatment with 15d‐PGJ 2 also significantly inhibited CASP‐induced lung inflammation and the production of pro‐inflammatory cytokines. Our results show that BMSCs can protect lung tissues from gastric aspiration injury and inhibit lung inflammation in rats. A beneficial effect might be achieved through BMSC‐derived 15d‐PGJ 2 activation of the PPAR‐γ receptor, reducing the production of proinflammatory cytokines.
Highlights
Gastric aspiration is a common clinical event
Many recent studies have reported the effects of bone-marrow-derived mesenchymal stem cells (BMSCs) on acute lung injury (ALI) [18, 19]
Few studies have investigated the effects of BMSCs on aspiration lung injury [20]
Summary
Gastric aspiration is a common clinical event. The content of the aspirate includes gastric contents, blood, bacteria, liquids and secretions. Gastric aspiration is a major direct cause of acute lung injury (ALI) and the more severe acute respiratory distress syndrome [1, 2]. Gastric aspiration may result in a variety of clinical lung injuries, ranging from mild, subclinical pneumonitis to a more severe, progressive disease with associated high mortality. Acute respiratory distress syndrome from gastric aspiration frequently occurs in an unconscious patient in one of every 2000–4000 anaesthetic cases [3, 4]. Acute respiratory distress syndrome associated with gastric aspiration accounts for approximately 30% of the mortality and 20% of all deaths attributable to anaesthesia [5]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
