Abstract
Dynamics of bone marrow cells number (BMC) in the primary culture isolated from young (3 months) and old (20 months) Wistar rats was investigated. Proliferative activity of BMC of old animals was 2 times higher than that of young animals in the primary culture. Such superiority of the proliferative activity of BMC in the primary culture obtained from old animals is associated with the ability to actively divide lymphocytes and longer “lifespan” of segmented neutrophils obtained from old animals. It should be noted, that the lymphocytes of young animals did not proliferate in the primary culture. The content of intracellular calcium in BMC in the cells of old animals was 3 times higher compared with cells of young animals, which revealed the relationship of intracellular calcium and proliferative activity of BMC. Induction of liver fibrosis led to an increase in the lymphocyte content in young animals by 167%, and in old ones only by 26%, while the lymphocytes of young animals acquired the ability to proliferate in the primary culture. It has been suggested that such differences in the behavior of BMC in primary culture obtained in young and old animals reflect differences in the BMC microenvironment of young and old animals, which leads to changes in the epigenetic-metabolic characteristics of BMC.
Highlights
If bone marrow cells number (BMC) were obtained from old animals, over the same time, under the same conditions, their number increased by 112%, i.e. almost 2 times more compared with young animals (Figure 1)
This was pronounced during the first 24 hours of cultivation; 2) such superiority in the proliferative activity of BMC in the primary culture obtained from old animals is associated with the active proliferation of bone marrow lymphocytes and a significant increase in “lifespan” in culture of segmented neutrophils compared with those of young animals; 3) in the bone marrow of old animals contained more lymphocytes compared with the bone marrow of young animals; 4) BMC contained 3 times more intracellular calcium compared with the cells of young animals; 5) induction of liver fibrosis in young and old animals, induced by repeated injections of copper sulfate, was accompanied by a pronounced age-dependent response to BMC
In young animals, fibrosis caused an increase in the number of lymphocytes in the bone marrow by 167%, and in old animals by 26%
Summary
These studies led to the formation of regenerative medicine, the prospects for which have high hopes [3] [4]. A sufficiently large amount of data was obtained They showed a therapeutic effect after transfusion of stem cells [9] [10]. The model of Cu-induced liver fibrosis showed a pronounced therapeutic effect after the administration of human embryonic stem cells to rats [11]. These studies suggest a decrease or “exhaustion” of the stem cell pool during ontogenesis. There are quite a large number of works in which it has been shown that the number of hematopoietic stem cells in the bone marrow decreases in the aging organism and more often than in young animals [12] [13] and other pathologies associated with the functions of immunocompetent cells and blood cells
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