Bone histomorphometry in diabetes mellitus.

Abstract

Diabetes mellitus (DM) is a common disease with many complications; recently, one more complication has been associated with DM, an increased risk of fragility fractures [1–3]. Both type 1 and type 2 diabetes patients display an increase in bone fragility, which appears to be somewhat independent of bone mineral density (BMD) [4]. In fact, type 2 diabetes (T2D) patients have, in general, normal BMD while type 1 patients (T1D) have low BMD, which may contribute to fracture risk, but does not fully account for it [5, 6]. In this regard, a study ana lyz ing bone mate r i a l s t r eng th (BMS) us ing microindentation testing in vivo, demonstrated that postmenopausal womenwith T2D had lower BMS, and therefore compromised bone material properties, which were negatively correlated with glycated hemoglobin [7]. This suggests that the excess fracture risk in diabetes may be related to poor bone quality, raising questions regarding bone structure, material properties, and remodeling activity in these patients. In addition, poor glucose control and hypoglycemia besides other features related to the diabetic patient such as impaired vision and balance due to retinopathy and neuropathy increase fall and fracture risk. [8]. There are just a few studies on bone histomorphometry in diabetes with only one study in T1D [9]. Armas et al. [9] performed histomorphometric and micro-CT measurements on 18 patients with T1D with a mean duration of disease of 15 years; 5 of the subjects had a history of fragility fractures. This cohort had reasonable glucose control with half of the subjects presenting with Hba1c levels lower than 7 %. Bone biopsies were compared with those from age-matched controls. Overall, there were no significant differences between patients and controls in the structural parameters measured by the two techniques. However, those patients with fractures had structural and dynamic trends different from the nonfracturing subjects by both methods of analysis. Their trabeculae were more Brod-like^ than Bplate-like^, presumably contributing to their bone fragility. In addition, fracturing patients demonstrated a trend towards lower bone remodeling parameters than those without fractures, but the only statistically significant difference was a lower mineralization lag time in T2D [10]. The first histomorphometric study of T2Dwas published in 1964 by Klein et al., who showed an increase of cortical area of the ribs [11]. In contrast, Leite Duarte and Silva [12] in 1996 demonstrated a significant decrease in cancellous and cortical structural parameters (e.g., bone volume, cortical thickness) in 26 diabetic patients (50–89 years of age) when compared to matched controls. These structural deficiencies were accompanied by a decrease in bone formation, as indicated by lower osteoblast surface. Furthermore, a study with 8 patients aged 37–67 years, 6 of whom had T2D and 2 had T1D showed that bone remodeling parameters (e.g., mineralizing surface, mineral apposition rate, and bone formation rate) were significant lower in T2D than controls, suggesting an osteoblast dysfunction [13]. More recently, Manavalan * C. A. Moreira carolina.aguiar.moreira@gmail.com