Abstract

Label-free chemical volumetric imaging is highly desired for biomedical applications and material science. Here, we present bond-selective intensity diffraction tomography (BS-IDT) based on the integration of mid-infrared photothermal microscopy with the computational imaging technique. BS-IDT demonstrates high-speed (up to ~6Hz) and large field of view (~100µmx100µm) chemical 3D imaging and enables the mid-infrared fingerprint spectra extraction with high fidelity. The superior performance of BS-IDT is validated by volumetric hyperspectral chemical imaging on cancer cells and Caenorhabditis elegans.

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