Abstract
BACKGROUND:In vitro generation of three-dimensional vessel network is crucial to investigate and possibly improve vascularization after implantation in vivo. This work has the purpose of engineering complex tissue regeneration of a vascular network including multiple cell-type, an extracellular matrix, and perfusability for clinical application.METHODS:The two electrospun membranes bonded with the vascular network shape are cultured with endothelial cells and medium flow through the engineered vascular network. The flexible membranes are bonded by amine-epoxy reaction and examined the perfusability with fluorescent beads. Also, the perfusion culture for 7 days of the endothelial cells is compared with static culture on the engineered vascular network membrane.RESULTS:The engineered membranes are showed perfusability through the vascular network, and the perfused network resulted in more cell proliferation and variation of the shear stress-related genes expression compared to the static culture. Also, for the generation of the complex vascularized network, pericytes are co-cultured with the engineered vascular network, which results in the Collagen I is expressed on the outer surface of the engineered structure.CONCLUSION:This study is showing the perfusable in vitro engineered vascular network with electrospun membrane. In further, the 3D vascularized network module can be expected as a platform for drug screening and regenerative medicine.
Highlights
A vascular bed is an intricate network of nutritive blood vessels that ramifies through the tissues of the body or of one of its parts
For the generation of the complex vascularized network, pericytes are co-cultured with the engineered vascular network, which results in the Collagen I is expressed on the outer surface of the engineered structure
The alginate vascular-network mold for a vascularized membrane was cross-linked onto the contact surface of a gelatin gel containing CaCl2 solution using a master mold made in advance with PDMS, and a vascular tree sacrificial mold was dried in the shape of the master mold
Summary
A vascular bed is an intricate network of nutritive blood vessels that ramifies through the tissues of the body or of one of its parts. The vascular network in the bed is vital for Natural tissue vascularization is a complex process and takes time that develops through vasculogenesis and angiogenesis in vivo [2, 3]. This work has the purpose of engineering complex tissue regeneration of a vascular network including multiple cell-type, an extracellular matrix, and perfusability for clinical application. METHODS: The two electrospun membranes bonded with the vascular network shape are cultured with endothelial cells and medium flow through the engineered vascular network. The perfusion culture for 7 days of the endothelial cells is compared with static culture on the engineered vascular network membrane. RESULTS: The engineered membranes are showed perfusability through the vascular network, and the perfused network resulted in more cell proliferation and variation of the shear stress-related genes expression compared to the static culture. The 3D vascularized network module can be expected as a platform for drug screening and regenerative medicine
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