Bombesin impairs spindle function in mitotic V79 Chinese hamster cells by a receptor-dependent mechanism

Abstract

Bombesin belongs to a family of peptides acting as local hormones with roles in growth regulation, neural function and secretion. Upon binding to its receptor bombesin primarily elicits an increase of inositolphosphates and diacylglycerol, events leading to increased [Ca 2+] i and activation of protein kinase C. When asynchronously growing V79 Chinese hamster cells were treated with bombesin in the 10 −9−10 −7 M concentration range their content of inositolphosphates increased and so did the frequency of mitotic cells with abnormal chromosomal arrangements (c-mitoses). Both effects were abolished by simultaneous addition of the synthetic peptide antagonist D-Arg 1,D-Phe 5,D-Trpu 9-Leu 11-substance P that binds to certain bombesin receptors. These results demonstrate that the V79 cells most probably have receptors for bombesin and that the weak but significant c-mitotic effect is mediated by such receptors.