Boer Spermatozoa Quality in Different Incubation Periods and Medium for In Vitro Fertilization (IVF) Preparation

Abstract

One of the developments in biotechnology is in vitro fertilization (IVF) requires a preparation process, one of it is spermatozoa. Preparation of spermatozoa is a stage of purification and separation as well as to eliminate bacteria and clear the unwanted residues. In preparation usually use a special medium to maintain the quality, as energy sources, and others. This research aims to determine the effect of incubation time on the quality of spermatozoa of Boer goats in two different types of medium, Tissue Culture Medium (TCM) -199 and Phosphate Buffer Saline (PBS). This research used 13 frozen semen obtained from Balai Besar Inseminasi Buatan (BBIB) Singosari then analysed the standart quality of post-thawing spermatozoa for next stages treatment. Post-thawing semen then prepared and tested in two types of media, there are TCM-199 and PBS. In each media, Semen was incubated at 38°C CO2 5% for 30 (P1), 45 (P2), and 60 (P3) minutes. The results of the study then observed sperm quality, like; motility, viability, abnormality, and concentration, in addition to quality, the effect of incubation time was analyzed using Randomized Block Design (RBD) and continued with further testing of the Least Significance Different (LSD). The results showed the average sperm motility for TCM-199 medium P1, P2, and P3 respectively; 28% ± 10.95, 34% ± 8.94, and 26% ± 5.48, while for PBS P1, P2, and P3 medium respectively; 58% ± 4.47, 56% ± 8.94, and 46% ± 8.94. The conclusion of this study is that PBS medium is better for maintaining sperm quality while incubation, especially in the 30 minute compared to TCM-199 medium.