Body Fluid Distribution in Rats With Cold-Induced Hypertension

Abstract

The purpose of this experiment was to determine body fluid distribution during chronic cold exposure and to further understand the mechanism of cold-induced hypertension. Blood pressures, hematocrit, and the plasma, blood, and extracellular fluid volumes were measured in rats at intervals of 1, 3, and 5 weeks after exposure to cold (5°C). Resting systolic, diastolic, and mean blood pressures measured by direct arterial cannula were significantly elevated in a time-dependent manner over the duration of cold exposure. The increase in diastolic blood pressure, which reflects the peripheral vascular resistance, exceeded that of systolic blood pressure after both 3 and 5 weeks of exposure to cold. Pulse pressure was significantly decreased by 3 and 5 weeks of cold exposure. The plasma, blood, and extracellular fluid volumes were significantly increased after both 1 and 3 weeks of exposure to cold, but had returned to control levels by 5 weeks of cold exposure. Cold exposure, however, did not affect the hematocrit. The 2-h water intake after the cold-exposed rats were returned to warm (25°C) (thermogenic drinking) was significantly increased compared to that of warm-acclimated rats during the first, third, and fifth week of exposure to cold. The present results suggest that the development of cold-induced hypertension is associated with blood volume expansion, and that the elevated blood pressure is maintained by increased peripheral vascular resistance without blood volume expansion. The results also imply that exposure to cold induces a dehydration in rats.