Abstract

The study utilised a pasture grazing based, voluntary traffic automatic milking system to investigate milk production of cows fed a pasture-based diet and supplemented with a pellet formulated with vs. without rumen-protected lysine and methionine (RPLM). The study adopted a switch-over design (over two periods of 5 and 10 weeks, respectively) and used 36 cows and equally allocated them into two experimental groups. The RPLM (Trial) pellet had 2% lower crude protein, but similar metabolizable energy content compared to the Control pellet. Pellet intake was 10.0 and 9.4 kg/day/cow. Milk yield was 36.2 and 34.4 kg/day/cow (p = 0.23), and energy corrected milk was 35.1 and 33.8 kg/day/cow (p = 0.076), and milk solids was 2.55 and 2.46 kg/cow/day (p = 0.073) in the Control and Trial groups, respectively. Milk fat%, milk protein%, milk fat: protein ratio, milking frequency and rumination time were not different between the two groups (p > 0.05). In period 1, plasma glucose was 3.1 mmol/L for both groups and milk urea were 150 and 127 mg/L in the Control and Trial groups, respectively. Both plasma glucose (as a proxy for energy supply) and milk urea (as a proxy for nitrogen use efficiency; NUE) were not different between groups (p > 0.05). This study showed that under a grazing pasture system, feeding lactating dairy cows a low protein pellet with RPLM supplementation, maintained milk production performance and NUE, compared with cows fed a high protein Control pellet diet with no RPLM. Further research should assess the long-term (seasonal) effects of feeding a diet formulated with RPLM on cow intake, health and reproductive performance.

Highlights

  • Methionine (Met) and lysine (Lys) are often the first two limiting amino acids (AA)in the lactating dairy cow diet [1,2,3]

  • In an earlier study, lactating dairy cows fed on a soybean meal-based diet supplemented with Met had a higher milk yield and milk protein yield than those ones fed with only soybean meal [6]

  • The formulated pellet with rumen-protected lysine and methionine (RPLM) had approximately 2% lower crude protein (CP), but similar metabolizable energy (ME) content compared to the Control pellet (Table 2)

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Summary

Introduction

In the lactating dairy cow diet [1,2,3]. The effects of supplementing dairy cows with Met and Lys were previously investigated in total mixed ration (TMR) systems, in European countries and United States [4,5]. In an earlier study, lactating dairy cows fed on a soybean meal-based diet supplemented with Met had a higher milk yield and milk protein yield than those ones fed with only soybean meal [6]. Lys and Met supplementation in lactating dairy cow diets stimulated feed intake, milk yield and milk protein%. There was limited or no improvement in feed intake and milk production performance in dairy cows supplemented with Met and Lys. For example, Rogers et al [1] showed that

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