Abstract
BackgroundAlmost all researchers agree on the lack of Bob-1 expression in Hodgkin/Reed-Sternberg (H/RS) cells in classic Hodgkin lymphoma (CHL), and utilize this marker as a diagnostic tool in conjunction with other markers to differentiate between lymphocyte predominance Hodgkin lymphoma (LPHL) and CHL.AimTo study the immunohistochemical (IHC) expression of Bob-1 in Egyptian CHL and to correlate this expression with Epstein-Barr virus (EBV) viral load.Materials and methodsParaffin sections of randomly selected 18 CHL cases were included: 2 lymphocyte rich (LR), 4 mixed cellularity (MC), 10 nodular sclerosis (NS) and 2 lymphocyte depletion (LD). All cases were immunostained for Bob-1. EBV was evaluated by EBV early RNA transcripts in situ hybridization (EBER ISH) and immunostaining for EBV latent membrane protein-1 (LMP-1).ResultsSixty seven percent of cases (12/18) were positive for EBV by ISH and/or immunostaining for LMP-1. Moderate to strong nuclear Bob-1 was observed in 94% of cases. The positivity ranged between 25–100%. Bob-1 immunoreactivity was strongly associated with EBV positivity (p < 0.001).ConclusionThis study proves nuclear IHC expression of Bob-1 on H/RS in CHL implying the difficulties in applying this marker to differentiate between LPHL and CHL. Does this difference between Western and Egyptian CHL reflect genetic and/or environmental factors, or simply no difference exists as most researchers are concentrated on the Western population and no comparative studies have been done. Studies from other countries might answer this question.
Highlights
Almost all researchers agree on the lack of Bob-1 expression in Hodgkin/ReedSternberg (H/RS) cells in classic Hodgkin lymphoma (CHL), and utilize this marker as a diagnostic tool in conjunction with other markers to differentiate between lymphocyte predominance Hodgkin lymphoma (LPHL) and CHL
This study proves nuclear IHC expression of Bob-1 on H/RS in CHL implying the difficulties in applying this marker to differentiate between LPHL and CHL
Does this difference between Western and Egyptian CHL reflect genetic and/or environmental factors, or no difference exists as most researchers are concentrated on the Western population and no comparative studies have been done
Summary
Almost all researchers agree on the lack of Bob-1 expression in Hodgkin/ReedSternberg (H/RS) cells in classic Hodgkin lymphoma (CHL), and utilize this marker as a diagnostic tool in conjunction with other markers to differentiate between lymphocyte predominance Hodgkin lymphoma (LPHL) and CHL. According to the World Health Organization (WHO) classification of haematological malignancies [1], the B-cell specific transcriptional co-activator or Bob-1/OCA-B is not expressed on H/RS cells, a point used to differentiate between CHL and LPHL, the latter being Bob-1 positive. Diagnostic Pathology 2007, 2:10 http://www.diagnosticpathology.org/content/2/1/10 of the latently expressed gene LMP-1 or on the detection of EBV early RNA transcripts (EBER) [7]. This work was undertaken to study the expression of Bob-1 in Egyptian CHL and to correlate this expression with EBV viral load in an attempt to find out if differences do exist between Western and Egyptian CHL, for optimal assessment of the treatment regime
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